Confocal Microscopy

Confocal microscopy is a technique for measuring surface texture and shape. In a confocal microscope, pinhole aperture is used to create a single focus point and to reject out-of-focus light, which enhances image resolution and contrast. An image is formed by scanning a laser source across the sample, generating a 2D image. These images are stacked to create a 3D map of the sample. Confocal microscopy is widely used in biology, medicine, and materials science,